abstract：:Transmission electron microscopy can be used to observe the ultrastructure of viruses and other microbial pathogens with nanometer resolution. In a transmission electron microscope (TEM), the image is created by passing an electron beam through a specimen with contrast generated by electron scattering from dense eleme...

journal_title：Journal of virological methods

authors： Monninger MK,Nguessan CA,Blancett CD,Kuehl KA,Rossi CA,Olschner SP,Williams PL,Goodman SL,Sun MG

更新日期：2016-12-01 00:00:00

abstract：:Cell culture based assays used to detect waterborne viruses typically call for incubating the sample for at least two weeks in order to ensure that all the culturable virus present is detected. Historically, this estimate was based, at least in part, on the length of time used for detecting poliovirus. In this study, ...

journal_title：Journal of virological methods

authors： Cashdollar JL,Huff E,Ryu H,Grimm AC

更新日期：2016-11-01 00:00:00

abstract：:Beak and feather disease virus (BFDV) threatens a wide range of endangered psittacine birds worldwide. In this study, we assessed a novel PCR assay and genetic screening method using high-resolution melt (HRM) curve analysis for BFDV targeting the capsid (Cap) gene (HRM-Cap) alongside conventional PCR detection as wel...

journal_title：Journal of virological methods

authors： Das S,Sarker S,Ghorashi SA,Forwood JK,Raidal SR

更新日期：2016-11-01 00:00:00

abstract：:Citrus tristeza virus (CTV) is the most economically important virus found on citrus and influences production worldwide. The 3' half of the RNA genome is generally conserved amongst sources, whereas the 5' portion is more divergent, allowing for the classification of the virus into a number of genotypes based on sequ...

journal_title：Journal of virological methods

authors： Kleynhans J,Pietersen G

更新日期：2016-11-01 00:00:00

abstract：:The plaque assay is essential for virion quantitation but the classic protocol requires considerable efforts. A simplified dengue 96-well plaque assay with automated quantitation program is an alternative to access the level of infectious virus. Dengue plaque assay was simplified using LLC/MK2 cells and virus mixing s...

journal_title：Journal of virological methods

authors： Boonyasuppayakorn S,Suroengrit A,Srivarangkul P,Yuttithamnon W,Pankaew S,Saelee T,Prompetchara E,Salakij S,Bhattarakosol P

更新日期：2016-11-01 00:00:00

abstract：:A one-step centrifugal ultrafiltration method was developed to enhance rapid detection of human enteric viruses and co-occurring viruses in wastewater. Samples were collected pre- and post-UV treatment at two full-scale tertiary municipal wastewater treatment plants in Calgary, Canada. Viruses were concentrated from 1...

journal_title：Journal of virological methods

authors： Qiu Y,Lee BE,Ruecker NJ,Neumann N,Ashbolt N,Pang X

更新日期：2016-11-01 00:00:00

abstract：:Highly pathogenic avian influenza virus H5N1 is a continuous threat to public health and poultry industry. The recurrence of the H5N1 led us to develop a robust, specific, and rapid detection method for the virus. In this study, an impedance aptasensor was developed for the virus detection using specific H5N1 aptamer ...

journal_title：Journal of virological methods

authors： Karash S,Wang R,Kelso L,Lu H,Huang TJ,Li Y

更新日期：2016-10-01 00:00:00

abstract：:The ongoing Zika virus (ZIKV) outbreak has rapidly spread to new areas of Americas, which were the first transmissions outside its traditional endemic areas in Africa and Asia. Due to the link with newborn defects and neurological disorder, numerous infected cases throughout the world and various mosquito vectors, the...

journal_title：Journal of virological methods

authors： Xu MY,Liu SQ,Deng CL,Zhang QY,Zhang B

更新日期：2016-10-01 00:00:00

abstract：:Disease caused by Chikungunya virus (CHIKV) is clinically characterized by sudden-onset of fever and severe arthralgia, which may persist for weeks, months, or years after acute phase of the infection. CHIKV is spreading globally; in India it first appeared in the 1960s followed by a quiescent period and then a full-b...

journal_title：Journal of virological methods

authors： Verma A,Chandele A,Nayak K,Kaja MK,Arulandu A,Lodha R,Ray P

更新日期：2016-09-01 00:00:00

abstract：:Conventional method to purify/concentrate dengue virus (DENV) is time-consuming with low virus recovery yield. Herein, we applied cellufine sulfate column chromatography to purify/concentrate DENV based on the mimicry between heparan sulfate and DENV envelope protein. Comparative analysis demonstrated that this new me...

journal_title：Journal of virological methods

authors： Kanlaya R,Thongboonkerd V

更新日期：2016-08-01 00:00:00

abstract：:In the last decades, molecular techniques have gradually been adopted for the rapid confirmation of results obtained through gold standard methods. However, international organisations discourage their use in routine laboratory investigations for rabies post-mortem diagnosis, as they may lead to false positive results...

journal_title：Journal of virological methods

authors： Aiello R,Zecchin B,Tiozzo Caenazzo S,Cattoli G,De Benedictis P

更新日期：2016-08-01 00:00:00

abstract：:Canine parvovirus (CPV) is an important disease causing gastroenteritis and/or haemorrhagic gastroenteritis in dogs. There are four antigenic types of CPV reported worldwide viz. CPV 2, CPV 2a, CPV 2b and CPV 2c. The diagnosis of CPV with the identification of the antigen type responsible remains problematic. In the p...

journal_title：Journal of virological methods

authors： Kaur G,Chandra M,Dwivedi PN,Narang D

更新日期：2016-07-01 00:00:00

abstract：:In the context of early infant diagnosis (EID) decentralization in sub-Saharan Africa, dried blood spot (DBS) is now widely used for HIV proviral DNA detection in resource-limited settings. A new version of CAP/CTM (version 2) has been introduced, recently by Roche Diagnosis as a new real-time PCR assay to replace pre...

journal_title：Journal of virological methods

authors： Gueye SB,Diop-Ndiaye H,Diallo MM,Ly O,Sow-Ndoye A,Diagne-Gueye ND,Kébé-Fall K,Diop F,Gaye-Diallo A,Belec L,Mboup S,Touré-Kane C

更新日期：2016-03-01 00:00:00

abstract：:HoBi-like pestiviruses are emerging pestiviruses that infect cattle causing clinical forms overlapping to those induced by bovine viral diarrhea virus (BVDV) 1 and 2. As a consequence of their widespread distribution reported in recent years, molecular tools for rapid discrimination among pestiviruses infecting cattle...

journal_title：Journal of virological methods

authors： Mari V,Losurdo M,Lucente MS,Lorusso E,Elia G,Martella V,Patruno G,Buonavoglia D,Decaro N

更新日期：2016-03-01 00:00:00

abstract：:A method for detection and identification of the hepatitis C virus antigen (HCVcoreAg) in human serum with consideration for possible amino acid substitutions is proposed. The method is based on a combination of biospecific capturing and concentrating of the target protein on the surface of the chip for atomic force m...

journal_title：Journal of virological methods

authors： Kaysheva AL,Ivanov YD,Frantsuzov PA,Krohin NV,Pavlova TI,Uchaikin VF,Konev VА,Kovalev OB,Ziborov VS,Archakov AI

更新日期：2016-03-01 00:00:00

abstract：:The fluorescent antibody test (FAT) is the most commonly used method for detection of the rabies virus (RV). The plaque assay can only be applied to fixed RVs, and cannot be used for street RVs. In this study, plaque formation allowing the determination of both fixed and street RVs was achieved using the immune plaque...

journal_title：Journal of virological methods

authors： Park JS,Um J,Choi YK,Lee YS,Ju YR,Kim SY

更新日期：2016-02-01 00:00:00

abstract：:To develop reverse genetics system of RNA viruses, cloning of full-length viral genome is required which is often challenging due to many steps involved. In this study, we report cloning of full-length cDNA from an Indian field isolate (CSFV/IVRI/VB-131) of classical swine fever virus (CSFV) using in vitro overlap ext...

journal_title：Journal of virological methods

authors： Kamboj A,Saini M,Rajan LS,Patel CL,Chaturvedi VK,Gupta PK

更新日期：2015-12-15 00:00:00

abstract：:Canine serum preserved at room temperature (25°C) for longer than 24h is known to exhibit significant cytotoxicity. This phenomenon is one of the major reasons for the failure of virus neutralization tests. In this study, a method for reducing this cytotoxicity was investigated by applying several treatments to dog, c...

journal_title：Journal of virological methods

authors： Shiraishi R,Hirayama N

更新日期：2015-12-01 00:00:00

abstract：:We assessed the performance of Abbott RealTime CMV assay (ARC) compared to Roche Cobas Amplicor CMV Monitor Test (RCM) for quantification of CMV in plasma of transplant patients. Commercial panels were used to test linearity, precision and interference and 83 clinical samples were used for the accuracy and precision a...

journal_title：Journal of virological methods

authors： Tremblay MA,Rodrigue MA,Deschênes L,Boivin G,Longtin J

更新日期：2015-12-01 00:00:00

abstract：:HPV-16 L1 methylation and E6/E7 mRNA have suggested that they had close relationship with cervical neoplastic progression. This study aimed to evaluate the clinical performance of the HPV-16 L1 methylation assay and E6/E7 mRNA test for detecting high-grade cervical lesions (CIN2+). A total of 81 women with liquid-base...

journal_title：Journal of virological methods

authors： Qiu C,Zhi Y,Shen Y,Gong J,Li Y,Rong S,Okunieff P,Zhang L,Li X

更新日期：2015-11-01 00:00:00

abstract：:A real-time RT-PCR assay based on the TaqMan technology was developed for rapid and sensitive detection of pestiviruses infecting cattle, i.e., bovine viral diarrhea virus (BVDV) 1, BVDV-2, and the emerging HoBi-like pestiviruses. The assay was linear and reproducible, being able to detect as few as 10 copies of viral...

journal_title：Journal of virological methods

authors： Losurdo M,Mari V,Lucente MS,Colaianni ML,Padalino I,Cavaliere N,Buonavoglia C,Decaro N

更新日期：2015-11-01 00:00:00

abstract：:Decapod Penstyldensovirus 1, previously named as infectious hypodermal and hematopoietic necrosis virus (IHHNV), is an economically important pathogen that causes shrimp diseases worldwide. However, a rapid method for cloning full-length IHHNV genome sequences is still lacking, which makes it difficult to study the ge...

journal_title：Journal of virological methods

authors： Wei YW,Fan DD,Chen J

更新日期：2015-11-01 00:00:00

abstract：:A new high-throughput GenomeLab Gene Expression Profiler (GeXP) analyser-based multiplex PCR assay was developed for the detection of eight reproductive and respiratory pathogens in swine. The reproductive and respiratory pathogens include North American porcine reproductive and respiratory syndrome virus (PRRSV-NA), ...

journal_title：Journal of virological methods

authors： Zhang M,Xie Z,Xie L,Deng X,Xie Z,Luo S,Liu J,Pang Y,Khan MI

更新日期：2015-11-01 00:00:00

abstract：:Detection of Hendra viral IgG antibody in animal sera is useful for surveillance following a virus outbreak. The commonly used enzyme-linked immunosorbent assay and fluorescence-based Luminex assay typically consist of three steps and take at least several hours to complete. We have simplified the procedure to two ste...

journal_title：Journal of virological methods

authors： Gao Y,Pallister J,Lapierre F,Crameri G,Wang LF,Zhu Y

更新日期：2015-09-15 00:00:00

abstract：:In situ detection of specific cells offers a unique perspective on the spatial interactions between host immune cells and specific viral pathogens or cancers. Most immunohistochemistry techniques require manual cell counting on biopsied and fixed tissue sections. The availability of sophisticated software packages for...

journal_title：Journal of virological methods

authors： Diem K,Magaret A,Klock A,Jin L,Zhu J,Corey L

更新日期：2015-09-15 00:00:00

abstract：:Group A rotaviruses (RVA) are important infectious agents associated with diarrhea in the young of several animal species including foals. Currently, a variety of diagnosis methods are commercially available, like ELISA, latex agglutination and immunochromatographic assays. These commercial tests are mainly designed f...

journal_title：Journal of virological methods

authors： Miño S,Kern A,Barrandeguy M,Parreño V

更新日期：2015-09-15 00:00:00

abstract：:Cervical carcinoma is one of the major consequences of human papillomavirus (HPV) infections. Although HPV infections of cervix do not always progress to cancer, 90% cases of cervical cancer have been found associated with high risk HPV (hrHPV) infection. Usually, HPV infection is asymptomatic; however, this asymptoma...

journal_title：Journal of virological methods

authors： Ramzan M,Noor ul Ain,Ilyas S,Umer M,Bano S,Sarwar S,Shahzad N,Shakoori AR

更新日期：2015-09-15 00:00:00

abstract：:Influenza reverse genetics plays vital roles in understanding influenza molecular characteristics and vaccine development. However, current influenza reverse genetics heavily depends on restriction enzyme and ligation for gene cloning. The traditional cloning process of influenza eight fragments for virus rescuing gen...

journal_title：Journal of virological methods

authors： Shao H,Fan Z,Wan Z,Tian X,Chen H,Perez DR,Qin A,Ye J

更新日期：2015-09-15 00:00:00

abstract：:Dengue virus (DENV) nonstructural-1 (NS1) glycoprotein is useful for diagnosis of DENV infections in the first 8 days of illness with any of the four serotypes (DENV-1, DENV-2, DENV-3 and DENV-4). However, NS1 diagnostics are less sensitive for secondary DENV infections so the utility of NS1 diagnostics in dengue ende...

journal_title：Journal of virological methods

authors： Gelanew T,Poole-Smith BK,Hunsperger E

更新日期：2015-09-15 00:00:00

abstract：:Phage-displayed random peptide libraries are widely used for identifying peptide interactions with proteins and other substrates. Selection of peptide ligands involves iterative rounds of affinity enrichment. The binding properties of the selected phage clones are routinely tested using immunoassay after propagation t...

journal_title：Journal of virological methods

authors： Hakami AR,Ball JK,Tarr AW

更新日期：2015-09-01 00:00:00

abstract：:Human cytomegalovirus (hCMV) is a ubiquitous pathogen that causes congenital infection and severe infections in immunocompromised patients. Chronic hCMV infection may also play an important role in immunosenescence and adverse health outcomes in older adults. THP-1, a human monocytic cell line and its derived macropha...

journal_title：Journal of virological methods

authors： Li H,Mao G,Carlson J,Leng SX

更新日期：2015-09-01 00:00:00

abstract：BACKGROUND:Polyomavirus BK (BKV) may cause nephropathy in renal transplant recipients and hemorrhagic cystitis in bone marrow recipients. We developed real-time PCRs (RT-PCR) to determine easily and rapidly the different BKV genotypes (BKGT) (I-IV). METHODS:On the VP1 gene a duplex of RT-PCRs was developed and validat...

journal_title：Journal of virological methods

authors： Gard L,Niesters HG,Riezebos-Brilman A

更新日期：2015-09-01 00:00:00

abstract：:A novel duck reovirus (N-DRV) disease emerged in China in 2000 and it has become an epidemic genotype. A test for detection of virus-specific antibodies in serum samples would be useful for epidemiological investigations. Currently, Currently, serological assays for N-DRV diagnosis are not available. A test for detect...

journal_title：Journal of virological methods

authors： Yun T,Chen H,Yu B,Zhang C,Chen L,Ni Z,Hua J,Ye W

更新日期：2015-08-01 00:00:00

abstract：:No effective prophylactic or therapeutic vaccine against HIV-1 in humans is currently available. This study analyzes the immunogenicity and safety of a recombinant attenuated vaccinia virus. A chimeric gene of HIV-1 multi-epitope genes containing CpG ODN and cholera toxin B subunit (CTB) was inserted into Chinese vacc...

journal_title：Journal of virological methods

authors： Du S,Wang Y,Liu C,Wang M,Zhu Y,Tan P,Ren D,Li X,Tian M,Yin R,Li C,Jin N

更新日期：2015-07-01 00:00:00

abstract：:Saint Louis encephalitis virus belongs to Flavivirus genus; Flaviviridae family jointly with other medically important flaviviruses including dengue virus and West Nile virus. The biological properties and functions of prM flavivirus protein are under investigation due to its importance in the generation of infectious...

journal_title：Journal of virological methods

authors： Pupo-Antúnez M,Vázquez S,Sosa AL,Caballero Y,Vásquez Y,Morier L,Álvarez M,Guzmán MG

更新日期：2015-06-15 00:00:00

abstract：:Porcine reproductive and respiratory syndrome virus (PRRSV) became a significant pathogen of swine upon its emergence in the late 1980s and since then has exemplified a rapidly evolving, constantly re-emerging pathogen. In addition to the challenges faced in development of vaccines and diagnostics, research on the bas...

journal_title：Journal of virological methods

authors： Spear A,Faaberg KS

更新日期：2015-06-15 00:00:00

abstract：:Bovine viral diarrhea virus (BVDV) should be a ubiquitous viral pathogen to the cattle and sheep industry. This pathogen is responsible for severe economic losses. We previously showed that plasmid-mediated dual short hairpin RNA (shRNA) efficiently inhibit BVDV replication in bovine kidney epithelial (MDBK) cells. In...

journal_title：Journal of virological methods

authors： Ni W,Qiao J,Ma Q,Wang J,Wang D,Zhao X,Cao Y,Li Q,Hu S,Chen C

更新日期：2015-06-15 00:00:00

abstract：:A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid detection of equine coronavirus (ECoV). This assay was conducted at 60 °C for 40 min. Specificity of the RT-LAMP assay was confirmed using several equine intestinal and respiratory pathogens in addition to ECoV. ...

journal_title：Journal of virological methods

authors： Nemoto M,Morita Y,Niwa H,Bannai H,Tsujimura K,Yamanaka T,Kondo T

更新日期：2015-04-01 00:00:00

abstract：:Bluetongue (BT) is an arboviral disease, which can often be fatal in naïve sheep and white tailed deer, but is usually less severe, or unapparent in other ruminants. Twenty-six bluetongue virus (BTV) serotypes have been recognised so far, two of which (BTV-25 and BTV-26) were recently identified by phylogenetic compar...

journal_title：Journal of virological methods

authors： Maan NS,Maan S,Belaganahalli M,Pullinger G,Montes AJ,Gasparini MR,Guimera M,Nomikou K,Mertens PP

更新日期：2015-03-01 00:00:00

abstract：:Dried blood spots (DBS) expedite the collection, storage and shipping of blood samples, thereby facilitating large-scale serologic studies. We evaluated the sensitivity of anti-HCV IgG testing and HCV-RNA quantitation using freshly prepared and stored DBS derived from HCV-infected patients. Protocols for elution were ...

journal_title：Journal of virological methods

authors： Tejada-Strop A,Drobeniuc J,Mixson-Hayden T,Forbi JC,Le NT,Li L,Mei J,Terrault N,Kamili S

更新日期：2015-02-01 00:00:00